IHC-Positive Hybridoma Screening

Creative Biolabs has accumulated extensive experiences in IHC-Positive Hybridoma Screening. For antibodies intended for immunohistochemical (or immunocytochemical) staining, in addition to the native immunogens, e.g. recombinant protein, we may alternatively use denatured antigens in animal immunization and hybridoma screening. Our theory is that antigens to be stained in IHC are denatured (completely or partially), altered (by cross-linking) or precipitated by paraffin embedding and various fixations. We may use fixative-treated immunogens in animal immunization. In the end, we perform hybridoma screening with IHC using paraffin-embedded slides or tissue microarrays at extra charges. Customer may provide the slides or tissue arrays; otherwise, we may provide the slides or arrays at customers' expense.